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Antigen
Requirement |
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0.5~2
mg of either protein or pre-conjugated antigen
in PBS for immunization of mice and additional
0.5 mg of un-conjugated antigen for ELISA.
Our Antibody service is broken into two phases:
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Phase
I: Immunization, bleed screening and fusion |
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Multiple
immunization of mice. |
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Tail
bleeds screening with ELISA and/or WB
if blots are provided by customer
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Send
data to customer for evaluation and let
customer to decide to proceed further or
terminate the project |
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Fusion
of tail bleed-positive mouse spleen |
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Phase
II: Clone Screening and Expansion
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Cloning
and screening by ELISA |
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Screening up to 20 ELISA-positive clones
by WB if blots are provided by the customer
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Report
data to customer for evaluation and let
customer choose up to 5 clones |
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Send
3 vials each of the selected hybridomas
producing antigen-specific antibodies
together with 5 ml culture supernatant
to customer
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Deliverables |
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Three
vials of frozen cells and 5 ml of culture
supernatants from clones (up to 5) of
hybridomas producing antigen-specific
monoclonal antibodies which meet the client's
needs.
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Simbiosys
manages a substantial portion of
risk inherent in any monoclonal
antibody development projects by
allowing the customers to terminate
the project at various points.
We recommended that customers discuss
their projects with our technical
staff. Please contact us at antibody@simbiosys-biowares.com
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