Metabolic Diseases and Inflammation

Several evidences now demonstrate a close link between metabolism and immunity. It is now known that obesity is associated with a state of chronic low-level inflammation. Simbiosys is interested in the molecular and cellular underpinnings of obesity-induced inflammation and the signaling pathways at the intersection of metabolism and inflammation that contribute to diabetes. Simbiosys offers ELISA-based assays to detect inflammation factors from compound treated cell lysates and blood samples (from in-vivo and clinical studies).

Factors Metabolic Regulation

Effects In-vitro Model
TNF-a in Obesity Promotes insulin resistance ELISA, macrophage
IL-6 in Obesity Promotes insulin resistance; Central anti-obesity action ELISA, hepatocytes
Leptin in Obesity Multiple effects on immune function; Suppresses appetite; Promotes FA oxidation ELISA, adipocytes
Adiponectin in Obesity Anti-inflammatory; Promotes insulin sensitivity; Stimulates FA oxidation ELISA, adipocytes
Resistin Variable in Obesity Induced in endotoxemia / inflammation; Promotes insulin resistance; Regulates fasting blood glucose level ELISA, adipocytes
IL-1 by hyperglycemia Proinflammatory; Regulates insulin secretion; Involved in central leptin action ELISA, macrophages & monocytes
IL-8 in Obesity Proatherogenic ELISA, monocytes
IL-10 in Obesity
in Metabolic syndrome
Anti-inflammatory; Promotes insulin sensitivity ELISA, lymphocytes
IL-18 in Obesity Proatherogenic ELISA, monocytes
MCP-1 in Obesity Proatherogenic; Promotes insulin resistance ELISA, monocytes
MIF in Obesity Inhibits macrophage migration ELISA, adipocytes
M-CSF Monocyte/macrophage differentiation; stimulates adipose growth ELISA, adipocytes
TGF- in Obesity Inhibits adipocyte differentiation and adipose tissue development; regulates atherosclerosis ELISA, blood serum
Soluble TNFR in Obesity Pro-inflammatory ELISA, blood serum
C-reactive Protein in Obesity Pro-inflammatory; atherogenic; risk factor for diabetes ELISA, blood serum
Cancer and Inflammation

A potential candidate for cancer causation is chronic inflammation. It is estimated that chronic inflammation accounts for 20% of all types of human cancers. Population studies have demonstrated the increased susceptibility of cells to become cancerous when exposed to chronic inflammation. Pathological links between inflammation and cancer is now well known but knowledge of the signal transduction pathways underlying them is at an early stage of development. Simbiosys is developing assaying solutions for the following inflammatory targets linked to cancer:

The inflammatory cytokine Tumor Necrosis Factor (TNF)-a is found in cancer biopsies and is associated with increased tumor grade. It is proposed that inhibiting TNF-a could block a primary cause of cancer

The Nuclear Factor (NF-kB) is proposed to be a pivotal protein in the link between inflammation and cancer. It is suspected that inhibiting NF-kB could halt tumor progression.


Assay Development
Cell Isolation & Banking
Cell Line Development
Natural Product Testing
Molecular Biology


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