Simbiosys will expand and store your
cell lines. Please contact us discuss the receipt
of your frozen cell lines and pricing. We offer the
following cell banking solutions:
Expansion
of cells for larger cryostorage lots
Expansion
of cell line for 20 vial storage
Expansion
of cell line for 25 vial storage
Expansion
of cell line for 30 vial storage
Viability
Testing per Cell Line
Preparation
of Master Cell Bank (MCB)
Expansion
of cell line in appropriate medium without
antibiotics
Pooling
of cells from culture vessel at time of
cryopreservation to create uniform suspension
of cells
Aliquoting
of >1.5 x 106 cells per vial into numbered
vials
Uniform
cryopreservation from room temperature
to liquid nitrogen over 24 hour period
Transfer
of cryopreserved cells to liquid nitrogen
tank
Random
selection of 3 vials for recovery rate and
viability
Preparation
of cryopreservation report
Preparation
of Working Cell Bank (WCB)
Re-establishment
of cell culture using cells from highest
number vial in MCB
